SEQUENCE FORMATBoth of the following examples could be accepted by SVMSignal:
Please input the protein sequence in FASTA format. Single-letter representation of 20 amino acids is accepted. Check your sequence before submitting to the program. The word following the character ">" is the title of the sequence.
MSRQSISLRF PLLLLLLSPS PVFSADPGAP APVNPCCYYP CQHQGICVRF GLDRYQCDCT
RTGYSGPNCT IPEIWTWLRT TLRPSPSFIH FMLTHGRWLW DFVNATFIRD TLMRLVLTVR
SNLIPSPPTY NIAHDYISWE SFSNVSYYTR ILPSVPRDCP TPMGTKGKKQ LPDAEFLSRR
FLLRRKFIPD PQGTNLMFAF FAQHFTHQFF KTSGKMGPGF TKALGHGVDL GHIYGDNLER
QYQLRLFKDG KLKYQMLNGE VYPPSVEEAP VLMHYPRGIP PQSQMAVGQE VFGLLPGLML
YATIWLREHN RVCDLLKAEH PTWGDEQLFQ TARLILIGET IKIVIEEYVQ QLSGYFLQLK
FDPELLFGAQ FQYRNRIAME FNQLYHWHPL MPDSFRVGPQ DYSYEQFLFN TSMLVDYGVE
ALVDAFSRQP AGRIGGGRNI DHHILHVAVD VIKESRVLRL QPFNEYRKRF GMKPYTSFQE
LTGEKEMAAE LEELYGDIDA LEFYPGLLLE KCHPNSIFGE SMIEMGAPFS LKGLLGNPIC
SPEYWKASTF GGEVGFNLVK TATLKKLVCL NTKTCPYVSF HVPDPRQEDR PGVERPPTEL
HOW TO SUBMIT
Click on SUBMIT to send your request and a confirmation page will appear. The page will refresh itself every 30 seconds and display the result when prediction is finished. Please remember your job submission URL. The prediction results will be stored online for 30 days and discarded thereafter.
Only top 100 residues and predicted states will display. The following is the description for the prediction result.
Position Protein sequence index Seq. Protein sequence SVMSignal The final predicted result from SVMSignal.
Label 's' means the residue is a part of the signal peptide.
Label 'C' means the last residue of the signal peptide.
Label 'L' means the residue is not a part of the signal peptide.
Cleavable The first level predicted result from SVMSignal.
Label 'C' means the potential cutting sites.
The calculated profile including free energy for convenience, amphiphilicity, charge index, polarity, and residue volume index will be drawn on the plane. The table also shows the physical-chemical properties of each residues.
The compressed file (SVMSignal.tar.gz) includes the AllPrediction.txt, AllCleavable.txt, and Profile.txt. If your query protein number is less than or equals to 500, the file will also include the profile images.